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normal sheep igg ctrl  (R&D Systems)


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    R&D Systems normal sheep igg ctrl
    Normal Sheep Igg Ctrl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+sheep+igg+ctrl/Normal+Sheep+IgG+Control/pm41712382-212-38-42
    Average 95 stars, based on 83 article reviews
    normal sheep igg ctrl - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Identification of endometrial CD169 + macrophages essential for Treg cell accumulation and implantation.
    Article Snippet: Intracellular Foxp3 staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience, Cat. No.00-5523-00). .. CD169+ macrophages were stained with a combination of the following antibodies: CD45.2-FITC (BioLegend, Cat. No. 109805), CD45.1-BV421 (BioLegend, Cat. No. 110731), CD45.1-PE (BioLegend, Cat. No. 110707), CD11b-PerCP/Cyanine5.5 (BioLegend, Cat. No. 101227), Sheep anti-CD169 (R&D Systems, Cat. No. MAB5610), Normal Sheep IgG Ctrl (R&D Systems, Cat. No. 5-001-A), AF647-conjugated donkey anti-sheep secondary antibody (Jackson ImmunoResearch, Cat# 713-605-003), F4/80-PE/Cy7 (BioLegend, Cat. No. 123113), CD64-PE (BioLegend, Cat. No. 139303), CX3CR1-PE (BioLegend, Cat. No. 149006), MerTK-PE (BioLegend, Cat. No. 151505), FOLR2-PE (BioLegend, Cat. No. 153304), Ly6C-PE (BioLegend, Cat. No. 128007), Ly6G-PE (BioLegend, Cat. No. 127607), MHC class 2-PE (BioLegend, Cat. No. 107607), Rat IgG2a,κIsotype Ctrl-PE (BioLegend, Cat. No. 400507), Mouse IgG1,κIsotype Ctrl-PE (BioLegend, Cat. No. 400111), Rat igG2b,κIsotype Ctrl-PE (BioLegend, Cat. No. 400607), Mouse igG2a,κIsotype Ctrl-PE (BioLegend, Cat. No. 400211), and Rat igG2c,κIsotype Ctrl-PE (BioLegend, Cat. No. 400707). .. Treg cells were stained with a combination of the following antibodies: CD25-BV421 (BioLegend, Cat. No. 102033), CD19-FITC (BioLegend, Cat. No. 115505), Helios-PE (BioLegend, Cat. No. 137206), Foxp3-APC (Invitrogen, Cat. No. 77-5775-40), CD4− PerCP/Cyanine5.5 (BioLegend, Cat. No. 100539), CD3-PE/Cyanine (BioLegend, Cat. No. 100219), CD45.2-PE/ Cyanine (BioLegend, Cat. No. 109829), F4/80-FITC (BioLegend, Cat. No. 123107), CD191 (CCR1)-APC (BioLegend, Cat. No. 152503), CD192 (CCR2)-APC (BioLegend, Cat. No. 150627), CD193 (CCR3)-APC (BioLegend, Cat. No. 144511), CD195 (CCR5)-PE (BioLegend, Cat. No. 107005), CD198 (CCR8)-BV605 (BioLegend, Cat. No. 150339), CD198 (CCR8)-BV605 (BioLegend, Cat. No. 150339), CD198 (CCR8)-BV605 (BioLegend, Cat. No. 150339), APC Rat IgG2b, κ Isotype Ctrl Antibody, (BioLegend, Cat. No. 400611), APC Rat IgG2a, κ Isotype Ctrl Antibody, (BioLegend, Cat. No. 400511), PE Armenian Hamster IgG Isotype Ctrl Antibody, 18 Cell Reports 45, 117040, March 24, 2026 (BioLegend, Cat. No. 400907), Brilliant Violet 605 Rat IgG2b, κ Isotype Ctrl Antibody (BioLegend, Cat. No. 400649), PE/Dazzle 594 antimouse/human Helios Antibody (BioLegend, Cat. No. 137231), Foxp3-PE (Invitrogen, Cat. No. 72-5775-40), CD3ε-BV785 (BioLegend, Cat. No. 100355), TCR γ/δ- PerCP/Cyanine5.5 (BioLegend, Cat. No. 118117), CD8a-APC/Fire 750 (BioLegend, Cat. No. 100765), NK1.1-BV 510 (BioLegend, Cat. No. 108737), CD25-BV605 (BioLegend, Cat. No. 102035), CD4-BV650 (BioLegend, Cat. No. 100545), CD19-AF594 (BioLegend, Cat. No. 115552), and Ki67-PE (BioLegend, Cat. No. 652404).

    Immunopeptidomics:

    Article Title: Identification of endometrial CD169 + macrophages essential for Treg cell accumulation and implantation.
    Article Snippet: Intracellular Foxp3 staining was performed using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience, Cat. No.00-5523-00). .. CD169+ macrophages were stained with a combination of the following antibodies: CD45.2-FITC (BioLegend, Cat. No. 109805), CD45.1-BV421 (BioLegend, Cat. No. 110731), CD45.1-PE (BioLegend, Cat. No. 110707), CD11b-PerCP/Cyanine5.5 (BioLegend, Cat. No. 101227), Sheep anti-CD169 (R&D Systems, Cat. No. MAB5610), Normal Sheep IgG Ctrl (R&D Systems, Cat. No. 5-001-A), AF647-conjugated donkey anti-sheep secondary antibody (Jackson ImmunoResearch, Cat# 713-605-003), F4/80-PE/Cy7 (BioLegend, Cat. No. 123113), CD64-PE (BioLegend, Cat. No. 139303), CX3CR1-PE (BioLegend, Cat. No. 149006), MerTK-PE (BioLegend, Cat. No. 151505), FOLR2-PE (BioLegend, Cat. No. 153304), Ly6C-PE (BioLegend, Cat. No. 128007), Ly6G-PE (BioLegend, Cat. No. 127607), MHC class 2-PE (BioLegend, Cat. No. 107607), Rat IgG2a,κIsotype Ctrl-PE (BioLegend, Cat. No. 400507), Mouse IgG1,κIsotype Ctrl-PE (BioLegend, Cat. No. 400111), Rat igG2b,κIsotype Ctrl-PE (BioLegend, Cat. No. 400607), Mouse igG2a,κIsotype Ctrl-PE (BioLegend, Cat. No. 400211), and Rat igG2c,κIsotype Ctrl-PE (BioLegend, Cat. No. 400707). .. Treg cells were stained with a combination of the following antibodies: CD25-BV421 (BioLegend, Cat. No. 102033), CD19-FITC (BioLegend, Cat. No. 115505), Helios-PE (BioLegend, Cat. No. 137206), Foxp3-APC (Invitrogen, Cat. No. 77-5775-40), CD4− PerCP/Cyanine5.5 (BioLegend, Cat. No. 100539), CD3-PE/Cyanine (BioLegend, Cat. No. 100219), CD45.2-PE/ Cyanine (BioLegend, Cat. No. 109829), F4/80-FITC (BioLegend, Cat. No. 123107), CD191 (CCR1)-APC (BioLegend, Cat. No. 152503), CD192 (CCR2)-APC (BioLegend, Cat. No. 150627), CD193 (CCR3)-APC (BioLegend, Cat. No. 144511), CD195 (CCR5)-PE (BioLegend, Cat. No. 107005), CD198 (CCR8)-BV605 (BioLegend, Cat. No. 150339), CD198 (CCR8)-BV605 (BioLegend, Cat. No. 150339), CD198 (CCR8)-BV605 (BioLegend, Cat. No. 150339), APC Rat IgG2b, κ Isotype Ctrl Antibody, (BioLegend, Cat. No. 400611), APC Rat IgG2a, κ Isotype Ctrl Antibody, (BioLegend, Cat. No. 400511), PE Armenian Hamster IgG Isotype Ctrl Antibody, 18 Cell Reports 45, 117040, March 24, 2026 (BioLegend, Cat. No. 400907), Brilliant Violet 605 Rat IgG2b, κ Isotype Ctrl Antibody (BioLegend, Cat. No. 400649), PE/Dazzle 594 antimouse/human Helios Antibody (BioLegend, Cat. No. 137231), Foxp3-PE (Invitrogen, Cat. No. 72-5775-40), CD3ε-BV785 (BioLegend, Cat. No. 100355), TCR γ/δ- PerCP/Cyanine5.5 (BioLegend, Cat. No. 118117), CD8a-APC/Fire 750 (BioLegend, Cat. No. 100765), NK1.1-BV 510 (BioLegend, Cat. No. 108737), CD25-BV605 (BioLegend, Cat. No. 102035), CD4-BV650 (BioLegend, Cat. No. 100545), CD19-AF594 (BioLegend, Cat. No. 115552), and Ki67-PE (BioLegend, Cat. No. 652404).



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    R&D Systems normal sheep igg ctrl
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    immunoglobulin g isotype controls for cgrp  (R&D Systems)
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    Fig. 6 ENT1−/− mice show neuroplastic changes in the cervical enlargement of the spinal cord at 6.5 months of age. A–C Representative images of immunofluorescent detection and quantification within the dorsal horn of the cervical spinal cord in wild-type and ENT1−/− mice. No differences were detected between left and right dorsal horns using Mann–Whitney’s test, so data were pooled (n = three to four female, three male per genotype). To account for anatomical and size differences in the region of interest, the upper, mid, and lower regions of the cervical enlargement were analyzed independently. A region of interest was defined around each dorsal horn to analyze laminae one to four, based on greyscale density using brightfield images. The area was standardized for each region of the cervical enlargement and the raw integrated density of immunoreactivity was averaged from up to three randomly selected sections from each region per animal. Immunoreactivity was measured for A calcitonin gene-related peptide <t>(CGRP),</t> B glial fibrillary acidic protein (GFAP), and C ionized calcium-binding adapter molecule 1 (IBA1). Data are plotted as mean ± SEM. *P < 0.05 by Mann–Whitney test for genotype differences and Kruskal–Wallis with Dunn’s multiple comparisons test for differences between cervical regions
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    Fig. 6 ENT1−/− mice show neuroplastic changes in the cervical enlargement of the spinal cord at 6.5 months of age. A–C Representative images of immunofluorescent detection and quantification within the dorsal horn of the cervical spinal cord in wild-type and ENT1−/− mice. No differences were detected between left and right dorsal horns using Mann–Whitney’s test, so data were pooled (n = three to four female, three male per genotype). To account for anatomical and size differences in the region of interest, the upper, mid, and lower regions of the cervical enlargement were analyzed independently. A region of interest was defined around each dorsal horn to analyze laminae one to four, based on greyscale density using brightfield images. The area was standardized for each region of the cervical enlargement and the raw integrated density of immunoreactivity was averaged from up to three randomly selected sections from each region per animal. Immunoreactivity was measured for A calcitonin gene-related peptide <t>(CGRP),</t> B glial fibrillary acidic protein (GFAP), and C ionized calcium-binding adapter molecule 1 (IBA1). Data are plotted as mean ± SEM. *P < 0.05 by Mann–Whitney test for genotype differences and Kruskal–Wallis with Dunn’s multiple comparisons test for differences between cervical regions
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    Image Search Results


    Fig. 6 ENT1−/− mice show neuroplastic changes in the cervical enlargement of the spinal cord at 6.5 months of age. A–C Representative images of immunofluorescent detection and quantification within the dorsal horn of the cervical spinal cord in wild-type and ENT1−/− mice. No differences were detected between left and right dorsal horns using Mann–Whitney’s test, so data were pooled (n = three to four female, three male per genotype). To account for anatomical and size differences in the region of interest, the upper, mid, and lower regions of the cervical enlargement were analyzed independently. A region of interest was defined around each dorsal horn to analyze laminae one to four, based on greyscale density using brightfield images. The area was standardized for each region of the cervical enlargement and the raw integrated density of immunoreactivity was averaged from up to three randomly selected sections from each region per animal. Immunoreactivity was measured for A calcitonin gene-related peptide (CGRP), B glial fibrillary acidic protein (GFAP), and C ionized calcium-binding adapter molecule 1 (IBA1). Data are plotted as mean ± SEM. *P < 0.05 by Mann–Whitney test for genotype differences and Kruskal–Wallis with Dunn’s multiple comparisons test for differences between cervical regions

    Journal: Arthritis research & therapy

    Article Title: Stiffness and axial pain are associated with the progression of calcification in a mouse model of diffuse idiopathic skeletal hyperostosis.

    doi: 10.1186/s13075-023-03053-3

    Figure Lengend Snippet: Fig. 6 ENT1−/− mice show neuroplastic changes in the cervical enlargement of the spinal cord at 6.5 months of age. A–C Representative images of immunofluorescent detection and quantification within the dorsal horn of the cervical spinal cord in wild-type and ENT1−/− mice. No differences were detected between left and right dorsal horns using Mann–Whitney’s test, so data were pooled (n = three to four female, three male per genotype). To account for anatomical and size differences in the region of interest, the upper, mid, and lower regions of the cervical enlargement were analyzed independently. A region of interest was defined around each dorsal horn to analyze laminae one to four, based on greyscale density using brightfield images. The area was standardized for each region of the cervical enlargement and the raw integrated density of immunoreactivity was averaged from up to three randomly selected sections from each region per animal. Immunoreactivity was measured for A calcitonin gene-related peptide (CGRP), B glial fibrillary acidic protein (GFAP), and C ionized calcium-binding adapter molecule 1 (IBA1). Data are plotted as mean ± SEM. *P < 0.05 by Mann–Whitney test for genotype differences and Kruskal–Wallis with Dunn’s multiple comparisons test for differences between cervical regions

    Article Snippet: Immunoglobulin G isotype controls for CGRP (1:750; 5–001-A, R&D systems: Minneapolis, MN), IBA1 (1:1000; 02–6102, Thermo Fisher Scientific), and GFAP (1:500; MA110,406, Thermo Fisher Scientific), as well as secondaryonly controls, were run in parallel.

    Techniques: Binding Assay, MANN-WHITNEY